Characterization of a New Green-Revertible Albino Mutant in Rice
2011
Wu, Liquan | Li, Ruiqing | Shu, Qingyao | Zhao, Haijun | Wu, Dianxing | Li, Juan | Wang, Rongfu
The rice (Oryza sativa L.) mutant G₉, isolated from ‘Guangzhan63S’ exposed to ⁶⁰Co γ irradiation in combination with in vitro tissue culture, exhibits albino and growth stage-dependent green-revertible phenotypes. The albino phenotype was observed in the mutant seedlings up to the fourth leaf (L4) stage, and then the seedlings entered into a transit period when old and newly extended leaves gradually revert to partial green. Finally, the leaves turn into full green at the sixth leaf (L6) stage. Chlorophyll abundance in the mutant seedlings at the second leaf (L2) stage was quite low and increased to about 1/4 of wild type Guangzhan63S at the L4 stage. At the L6 stage, the levels of chlorophyll in G₉ were almost identical to that in Guangzhan63S. Ultra-microstructure analysis showed that the G₉ mutant had no chloroplast at the L2 stage and fewer and smaller undifferentiated plastids at the L4 stage than Guangzhan63S at the same stage; no significant difference in chloroplast structure was found between G₉ and Guangzhan63S at the L6 stage. We also performed the simple sequence repeat (SSR) haplotype analysis to map the gene(s) responsible for the albino phenotype. Our data revealed that the green-revertible albino trait of G₉ was controlled by a single recessive nucleic gene, named clbGZ, located between the indel markers of IDJ6 (0.16 cM) and IDJ12 (0.25 cM) on the rice chromosome 11.
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