Anticandida activity of various Artemisia mesatlantica M. extracts

Ghazi, S.; Rouani, M.; Sekkat, Z. L.; Il Idrissi, A.; Arahou, M.; Hassikou, R.

Anticandida activity of various Artemisia mesatlantica M. extracts | Activité anticandidosique de divers extraits d’Artemisia mesatlantica M

2016

The blue wormwood (Artemisia mesatlantica M.), endemic in Morocco, is commonly used in traditional medicine for diseases treatment of the digestive and genital systems and dermatological infections....However, this therapeutic aspect and the active constituents of this plant are very few mentioned.This work has set an objective to propose an alternative to current fungal treatments that are becoming increasingly ineffective against resistant strains, in particular Candida albicans, species commonly found in fungal infections.Thus, three extracts obtained by maceration in solvents with increasing polarity (hexane, dichloromethane and methanol) and an aqueous extract by decoction were tested against five references strains. A serial dilution has been established by a geometric connection at the rate of 1/2 so as to obtain concentrations of 50 mg/ml to 0.095 mg ml. The method of microtitration has enabled to determine the minimum inhibitory concentrations (MIC). A phytochemical study of the dichloromethane extract was performed by combination of chromatographic and spectroscopic methods. The methanolic and aqueous extracts are the most active. However, the hexane extracts and dichloromethane have manifested fungicidal action. The reference synthetic products were also tested at licensed doses and globally these products are proven ineffective. Phytochemical analysis revealed the presence of sesquiterpene lactones and flavonoids.

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