Ammonia and pH effects on some metabolic parameters and gill histology of silver catfish, Rhamdia quelen (Heptapteridae)
2008
Miron, Denise dos S. | Moraes, Bibiana | Becker, Alexssandro G. | Crestani, Márcia | Spanevello, Rosélia | Loro, Vania L. | Baldisserotto, Bernardo
The aim of the present study was to assess the effect of ammonia exposure at different pH on survivorship and metabolic parameters in the liver, muscle and gill histology of silver catfish (Rhamdia quelen). The 96 h-LC50 of un-ionized ammonia (mg L⁻¹) at pH 6.0, 7.5 and 8.2 were: 0.44 (C.I. 0.38-0.49), 1.45 (C.I. 1.25-1.65) and 2.09 (C.I. 1.85-2.36), respectively. Survival of juveniles exposed to different ammonia levels was altered by pH, and fish exposed to all ammonia levels and different pH showed muscle glucose, muscle and liver glycogen reduction. Liver glucose and muscle and liver lactate levels increased in all fish exposed to ammonia as compared to the control. Exposure to waterborne ammonia increased total ammonia levels in both tissues and also induced gill epithelium damages such as lamellar fusion and edema as compared with controls at different pH. Silver catfish exposed to pH 6.0 and different NH₃ levels presented significantly higher hepatic glucose and protein levels when compared to those maintained at low NH₃ levels. Juveniles exposed to NH₃ levels at pH 7.5 and 8.2 showed lower hepatic protein levels compared to those maintained at low NH₃ levels. These parameters are indicative of pH dependence on ammonia toxicity in silver catfish. The metabolic parameters and gill histology may be used as early indicators of ammonia toxicity in silver catfish.
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