Transcript profiles of genes expressed in endosperm tissue are altered by high temperature during wheat grain development
2004
Altenbach, S.B. | Kothari, K.M.
Timing of transcript accumulation for genes involved in a variety of cellular processes was assessed by RT-PCR in endosperm from developing wheat grains grown under moderate (24/17 degrees C day/night) and high (37/28 degrees C day/night) temperature regimens. Under moderate temperatures, transcripts for proteins with storage functions were present at all five time points examined between 7 and 34 DPA, while transcripts for proteins involved in signal transduction, protein synthesis and metabolism were most abundant from 7 to 20 DPA. Transcripts for proteins that play roles in defense were present from 14 DPA, about the time that starch accumulation commenced, to 34 DPA. High temperatures advanced and compressed the timing of transcript accumulation during grain development. Comparisons of transcript profiles with the timing of key events in grain development identified genes whose transcripts were accumulated at equivalent stages under the two temperature regimens and might serve as markers for grain development. These comparisons also revealed a number of genes with transcript profiles that were shifted under high temperatures in a manner that was not consistent with developmental events. These genes may be involved in responses to high temperature that are distinct from effects on the timing of developmental processes.
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