Production and characterization of monoclonal antibodies to bovine beta2-microglobulin

Guidry, A.J.; Shapiro, R.P.; Srikumaran, S.; Nickerson, S.C.; Goldsby, R.A.

Production and characterization of monoclonal antibodies to bovine beta2-microglobulin

1988

Guidry, A.J. | Shapiro, R.P. | Srikumaran, S. | Nickerson, S.C. | Goldsby, R.A.

In an attempt to isolate monoclonal antibodies specific for bovine lymphocytes, spleen cells from mice immunized with bovine lymphocytes were fused with the mouse myeloma cell line SP-2/0. The resulting hybridoma cell lines were tested for reactivity with bovine lymphocytes, polymorphonuclear neutrophils, RBC, gamma-globulin, kappa-casein, beta-casein, alpha-S1-casein, and beta2-microglobulin (beta2m) and with beta2m from rabbits, goats, and human beings. None of the clones secreted anti-bovine lymphocyte-specific antibody. However, 4 secreted monoclonal antibodies to bovine beta2m. They also reacted with beta2m from rabbit, goat, and human being. One monoclonal antibody also was found to be reactive with bovine immunoglobulin. Monoclonal antibodies to beta2m could serve as a tool to (1) exlore the homology of the beta2m molecule among various species, (2) examine the relationship of beta2m with the constant region of the immunoglobulin molecule, (3) quantitate bovine beta2m in various body fluids and major histocompatibility antigens on cell surfaces, (4) help characterize those antigens in cattle, and (5) be used for tissue typing of those antigens.

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