The effect of mild cold exposure on UCP3 mRNA expression and UCP3 protein content in humans
2002
Schrauwen, P. | Westerterp-Plantenga, M.S. | Kornips, E. | Schaart, G. | Marken Lichtenbelt, W.D van
OBJECTIVE: In rodents, adaptive thermogenesis in response to cold exposure and high-fat feeding is accomplished by the activation of the brown adipose tissue specific mitochondrial uncoupling protein, UCP1. The recently discovered human uncoupling protein 3 is a possible candidate for adaptive thermogenesis in humans. In the present study we examined the effect of mild cold exposure on the mRNA and protein expression of UCP3. SUBJECTS: Ten healthy male volunteers (age 24.4±1.6 y; height 1.83±0.02 m; weight 77.3±3.0 kg; percentage body fat 19±2) DESIGN: Subjects stayed twice in the respiration chamber for 60 h (20.00-8.00 h); once at 22°C (72°F), and once at 16°C (61°F). After leaving the respiration chamber, muscle biopsies were taken and RT-competitive-PCR and Western blotting was used to measure UCP3 mRNA and protein expression respectively. RESULTS: Twenty-four-hour energy expenditure was significantly increased at 16°C compared to 22°C (P<0.05). At 16°C, UCP3T (4.6±1.0 vs 7.7±1.5 amol/µg RNA, P=0.07), UCP3L (2.0±0.5 vs 3.5±0.9 amol/µg RNA, P=0.1) and UCP3S (2.6±0.6 vs 4.2±0.7 amol/µg RNA, P=0.07) mRNA expression tended to be lower compared with at 22°C, whereas UCP3 protein content was, on average, not different. However, the individual differences in UCP3 protein content (16-22°C) correlated positively with the differences in 24 h energy expenditure (r=0.86, P<0.05). CONCLUSION: The present study suggests that UCP3 protein content is related to energy metabolism in humans and might help in the metabolic adaptation to cold exposure. However, the down-regulation of UCP3 mRNA with mild cold exposure suggests that prolonged cold exposure will lead to lower UCP3 protein content. What the function of such down-regulation of UCP3 could be is presently unknown.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل National Agricultural Library