Profile of the responsiveness of corpora allata from virgin female Periplaneta americana to an allatostatin from Diploptera punctata
1991
Weaver, R.J.
Radiochemical measurements of juvenile hormone III biosynthesis by isolated corpora allata from virgin adult female Periplaneta americanahave been used to investigate the in vitro sensitivity and pharmacodynamic response of theseendocrine glands to a synthetic preparation of an allatostatin from a distantly related cockroach species. The action of this peptide [allatostatin-1 = APSGAQRLYGFGL-amide] from Diplopterapunctata towards suitably responsive corpora allata from P. americana was found to berapid, reversible and dose-dependent. The neurohormone exerted its full effect withinapprox. 20 min from the start of an in vitro incubation and could be washed out in less than 1h. The dose-dependency covered the limited range 10-(9)-10-(6) M with an ED50 of approx.6.9 X 10-(8)M. A study of the inhibitory responses of corpora allata from virgin female P.americana of different ages revealed a clearly-defined developmental pattern of allatostatin sensitivity. It is suggested that the procedures for isolating similar such compounds from P.americana will have been simplified by this clear demonstration of both sensitive andinsensitive periods of corpora allata responsiveness to what may be a closely relatedallatostatic peptide.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل National Agricultural Library