Development and validation of microsatellite markers for Sargassum fusiforme based on transcriptomic data
2022
Jia, Rujie | Lu, Xiaoping | Wang, Wenjun | Liang, Zhourui | Yao, Haiqin | Li, Baoxian
The purpose of this study was to develop stable microsatellite markers and evaluate the genetic background of cultivated Sargassum fusiforme. Based on the transcriptome data obtained by high-throughput sequencing, eleven polymorphic microsatellite markers were developed using four S. fusiforme populations from China. One cultivated population was from Dongtou (DT) and three wild populations were from Muye Island (MY), Pingyu Island (PY) and Nanji Island (NJ). The cultivated S. fusiforme had the highest genetic diversity, with 90.91% polymorphic loci and Shannon’s information index (I) of 0.606, which were much higher than those of the wild populations (I = 0.425). The four populations were divided into two groups through a structure analysis. DT, PY and NJ were clustered into a group, and MY was an almost completely separate group. Both the structure and principal coordinates analysis showed that DT and PY had the lowest genetic differentiation, suggesting that the parents of the breeding population in Dongtou partly come from Pingyu Island. The observed heterozygosity of NJ was much higher than expected, indicating that the NJ wild resource was greatly affected by the environment. These eleven microsatellite markers could provide additional markers for germplasm resource evaluations and facilitate genetic analyses of the S. fusiforme population.
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