Distribution of rotenone and deguelin in Tephrosia vogelii and separation of rotenoid-rich fractions
1970
Delfel, N.E. | Tallent, W.H. | Carlson, D.G. | Wolff, I.A.
Rotenone and deguelin were determined by thin-layer densitometry in seven varieties of Tephrosia vogelii. The leaflets contained more deguelin than rotenone; the reverse was generally found in the petioles, stems, and roots. One variety contained deguelin but no rotenone. In other varieties the leaflets had more rotenone than other parts of the plant, and several times as much deguelin. Fractionation to recover rotenoid-rich leaflets was possible because they were more friable than stem portions of partially dried plants. Minimizing drying reduced rotenoid destruction. Separation by a whizzer-type air classifier was preferred over hammer or disc mills followed by sieving for three reasons: it was operable with higher moisture material (leaflet moisture content 12-14%), so rotenoid loss during drying was reduced; it avoided additional losses possibly caused by frictional heating in the mills; and it gave virtually complete (up to 97%) separation of leaflets from stem-petiole fractions.
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