Effects of temple particles on inflammation and endothelial cell response
2012
Lin, Lian-Yu | Lin, Hui-Yi | Chen, Hua-Wei | Su, Deli | Huang, Li-Chu | Chuang, Kai-Jen
To pray in temples is a regular activity in Buddhism and Taoism societies, yet few studies investigated the effects of particles from incense-burning in temples. The objectives of this study are to examine particle size and polycyclic aromatic hydrocarbon (PAH) effects of particles on coronary artery endothelial cell. We used two micro-orifice uniform deposit impactors to collect 11 sets of particles at a Chinese temple in Yi-Lan, Taiwan. 16 PAHs were determined by a high-resolution gas chomatograph/high-resolution mass spectrometer. Human coronary artery endothelial cells were exposed to particle extracts in three size ranges: PM₀.₁ (diameters less than 0.1μm), PM₁.₀–₀.₁ (diameters between 1.0 and 0.1μm), and PM₁₀–₁.₀ (diameters between 10 and 1.0μm) at 50μg/mL for 4h, and interleukin-6 (IL-6), endothelin-1 (ET-1), and nitric oxide (NO) concentrations in the medium were measured. We found that PM₁.₀–₀.₁ stimulation resulted in significantly higher IL-6 and ET-1 production than PM₀.₁ and PM₁₀–₁.₀. PM₁.₀–₀.₁ also significantly reduced HCAEA cells to synthesize NO. Naphthalene, acenaphthylene, acenaphthene and anthracene of PM₁.₀–₀.₁ were highly correlated with NO reduction. This study found that size and composition of temple particles were both important factors in inducing cytokine production and reducing NO formation in human coronary artery endothelial cell cultures.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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