Highly selective and sensitive visualization and identification of glycoproteins using multi-functionalized soluble dendrimer
2017
Li, Tiantian | Yu, Zhenlong | Zhang, Liyuan | Wang, Chao | Deng, Sa | Huo, Xiaokui | Tian, Xiangge | Zhang, Baojing | Ma, Xiaochi
Glycoproteins are the most important and complex group of posttranslational modifications known in proteins. Many clinical biomarkers and therapeutic targets in cancer are glycoproteins. However, the isolation of glyco-specific antibodies and their poor stability remains a significant challenge in analytical method and diagnostic development. In this work, for the first time, we present a technology for highly efficient and selective glycosylation analysis on membrane without the use of glyco-specific antibodies. This approach, termed Nanopoly-BAV, which uses polyamidoamine dendrimers multifunctionalized with boronic acid for specific binding to glycoproteins and with biotin groups for glycoproteins visualization. The Nanopoly-BAV confers femtomolar sensitivity, exceptional glycoprotein specificity and selectivity with as high as 100000 folds for glycoproteins over nonglycoproteins. This synthetic, robust and highly selective Nanopoly-BAV has a great potential to measure cell signaling events by clearly distinguishing actual glycosylation signals from protein expression changes with superior stability. This technique may provide a powerful tool to monitor cellular signaling pathways and discovering new signaling events.
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