Mass-produced Spirulina-mediated altered responses in ARPE-19 and HaCaT cells for biomedical applications
2022
Jin, Su-Eon | Lee, Sung Jae | Park, Cheon-Young
Spirulina has been highlighted for potential health benefits based on its therapeutic functions. In the present study, the physicochemical characteristics and effects of Spirulina on ARPE-19 and HaCaT cells were monitored after mass-production, compared with conventional product. Gene ontology (GO) was analyzed using systems biology network that accounted for the function of Spirulina in human health. Spirulina powder had a smooth surface with adsorbing particles and contained sodium, magnesium, and potassium. Spirulina powder extracts in 0.1 × PBS, 0.1 M HEPES, 0.1 M NaCl, ethanol, isopropyl alcohol, and acetonitrile showed broad absorbance peaks at 300–450 and 500–700 nm. In ARPE-19 and HaCaT cells, Spirulina powder in PBS displayed proliferation at 2–20 mg/mL and 2 mg/mL, respectively. Cell viability levels of HaCaT cells were almost higher than 90% at 0.1–20 mg/mL. Despite cell susceptibility, phycocyanin, which is a component of Spirulina, helped maintain consistency of cell responses with Spirulina. Spirulina functions were explained by GO-terms, mainly binding, cellular process, and cell in the network, in terms of anti-oxidant activity. Mass-produced Spirulina powder can be developed as a high-value nutraceutical for therapeutic applications in clinics. This protocol can be extended to other materials for biomedical applications.
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