Data on final calcium concentration in native gel reagents determined accurately through inductively coupled plasma measurements
2016
Viviano, Jeffrey | Wu, Hao | Krishnan, Anuradha | Ramanujachary, Kandalam | Venkataraman, Venkat
In this article we present data on the concentration of calcium as determined by Inductively Coupled Plasma (ICP) measurements. Calcium was estimated in the reagents used for native gel electrophoresis of Neuronal Calcium Sensor (NCS) proteins. NCS proteins exhibit calcium-dependent mobility shift in native gels. The sensitivity of this shift to calcium necessitated a precise determination of calcium concentrations in all reagents used. We determined the calcium concentrations in different components used along with the samples in the native gel experiments. These were: 20mM Tris pH 7.5, loading dye and running buffer, with distilled water as reference. Calcium determinations were through ICP measurements. It was found that the running buffer contained calcium (244nM) over the blank.
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