Introgression of perennial teosinte genome into maize and identification of genomic in situ hybridization and microsatellite markers
2005
Tang, Q. | Rong, T. | Song, Y. | Yang, J. | Pan, G. | Li, W. | Huang, Y. | Cao, M.
To transfer desirable characters from perennial teosinte [Zea perennis (Hitchc.) Reeves & Mangelsd.] into maize (Z. mays L.), we have generated an interspecific hybrid and its backcross generations (BC1F3). The maize x perennial teosinte BC1F3 (MZI202) resembled maize, with chromosome number 2n = 20. MZI202 was studied by multicolor genomic in situ hybridization (McGISH) and simple-sequence repeat (SSR) microsatellites markers. The McGISH experiments provided direct evidence that MZI202 was a maize-perennial teosinte substitution line with introgression of three alien chromosomes from perennial teosinte. The SSR assay further confirmed that a single chromosome 6 and the pair of chromosome 10 of maize were replaced by perennial teosinte chromosomes in MZI202.
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