Identification of a variant of Fusarium proliferatum that hydrolyzes fumonisin B1
1998
Keller, S.E. | Sullivan, T.M.
Fumonisins are mycotoxins produced primarily by Fusarium moniliforme and Fusarium proliferatum in corn. Normally, the fumonisins display considerable chemical stability at temperatures up to 100 degrees C at neutral pH and are stable in a pH range of 4-10 at ambient temperature. Fumonisin B1 (FB1) can be produced in bioreactors using F. proliferatum (M5991). Recently, some bioreactor-grown cultures of F. proliferatum were found to rapidly hydrolyze FB1. Subcultures from stock frozen from these FB1-degrading variants continued to degrade FB1 when grown under the same bioreactor conditions. The initial hydrolysis was catalyzed by an esterase and resulted in the appearance of the aminopentol backbone (AP1) of FB1. A cell-free extract retaining the FB1 hydrolytic activity could be obtained by extraction of cell paste with 10% Triton X-100. Activity could be destroyed by heating to 60 degrees C for 5 min or by the addition of acetonitrile.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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