Enzymatic transformation of aflatoxin B1 by Rh_DypB peroxidase and characterization of the reaction products
2020
Loi, Martina | Renaud, Justin B. | Rosini, Elena | Pollegioni, Loredano | Vignali, Elisa | Haidukowski, Miriam | Sumarah, Mark W. | Logrieco, A. | Mulè, Giuseppina
In some environments, a number of crops, notably maize and nuts can be contaminated by aflatoxin B₁ and related compounds resulting from the growth of aflatoxin-producing Aspergilli. Fungal peroxidases have been shown to degrade a number of mycotoxins, including aflatoxin B₁ (AFB₁). Therefore, the purpose of this study was to investigate the in vitro enzymatic degradation AFB₁ by a recombinant type B dye decolorizing peroxidase (Rh_DypB). Analysis of the reaction products by HPLC-MS analysis showed that under optimized conditions AFB₁ was efficiently transformed by Rh_DypB, reaching a maximum of 96% conversion after 4 days of reaction at 25 °C. Based on high resolution mass spectrometry analysis, AFB₁ was demonstrated to be quantitatively converted to AFQ₁, a compound with a significantly lower toxicity. A number of low molecular mass compounds were also present in the final reaction mixture in small quantities. The results presented in this study are promising for a possible application of the enzyme Rh_DypB for aflatoxin reduction in feed.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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