Pharbitis class-1 knotted-like homeobox gene, PKn3, shares similar characteristics to those of class-2 knotted-like genes
2000
Kobayashi, A. | Kiyosawa, N. | Suzuki, Y. | Murofushi, N. | Yamaguchi, I.
Three novel homeobox genes, PK(n)1-3 (Pharbitis knotted-like), were isolated from Japanese morning glory (Pharbitis nil Chois, strain Violet). A sequence analysis showed that these genes belong to the knotted class-1 gene family and that PK(n)3 has a relatively unique sequence. All PK(n) genes are expressed in shoot apices, stems and roots, but not in cotyledons. Transformed tobacco with PK(n)1 or PK(n)2 displayed leaf shrinkage and a dwarf phenotype, while the ectopic expression of PK(n)3 gave no altered phenotypes. In situ hybridization showed that PK(n)3 is up-regulated in developing leaf primordia and that this expression becomes restricted in the basal region of young leaf blades, which is reminiscent of the expression pattern of the class-2 knotted gene, NTH23. These data suggest that these Pharbitis homeobox genes participate in the differentiation in shoots and suggest a unique function of PK(n)3 in developing leaves.
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