Inactivation of the ftsH gene of Lactobacillus plantarum WCFS1: Effects on growth, stress tolerance, cell surface properties and biofilm formation
2012
Bove, Pasquale | Capozzi, Vittorio | Garofalo, Carmela | Rieu, Aurelie | Spanò, Giuseppe | Fiocco, Daniela
FtsH proteins are ubiquitous membrane-bound, ATP-dependent metalloproteases of the AAA family. In eubacteria, FtsH is involved in protein quality control under stress conditions. Lactobacillus plantarum is a widespread lactic acid bacterium that is encountered in several fermented food, including dairy products, vegetables and meat. In the present work the expression of the ftsH gene of L. plantarum was studied by quantitative real time RT-PCR in bacterial cultures subjected to various abiotic stresses. Both oxidative stress and addition of a membrane-fluidizing agent induced ftsH transcription, while a depletion of carbon-source repressed its mRNA level. Mutants deprived of the FtsH protease exhibited remarkable sensitivity to elevated temperature and increased salt concentration; conversely, overexpression of ftsH resulted in increased thermotolerance and resistance to salt. FtsH mutant had a reduced capacity to form biofilms on abiotic surfaces and exhibited different cell surface physico-chemical properties with respect to the wild type strain.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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