Improved activity and stability of pseudomonas capaci lipase in a novel biocompatible ionic liquid, 1-isobutyl-3-methylimidazolium hexafluorophosphate
2008
BACKGROUND: Enzymes may exhibit enhanced activity, stability and selectivity in ionic liquids, depending on the properties of the liquid. The physical-chemical properties of ionic liquids, however, may be modified by altering the anion or cation in the ionic liquid. This feature is a key factor for realizing successful reactions. In this work, a new ionic liquid, 1-isobutyl-3-methylimidazolium hexafluorophosphate (abbreviated as [i-C₄mim][PF₆]), was synthesized and investigated as a novel medium for the transesterification reaction of 2-phenylethanol with vinyl acetate catalyzed by pseudomonas capaci lipase. As contrasts, the reaction was also carried out in two reference solvents; the isomeric ionic liquid [i-C₄mim][PF₆], 1-butyl-3-methylimidazolium hexafluorophosphate (abbreviated as [C₄mim][PF₆]), and hexanes.RESULTS: As reaction medium, [i-C₄mim][PF₆] was best among the three solvents. The initial reaction rate, the equilibrium conversion of 2-phenylethanol and the half-lifetime of the lipase in [i-C₄mim][PF₆] medium were about 1.5, 1.2 and 3-fold that obtained in [C₄mim][PF₆] medium, respectively. The lipase in [i-C₄mim][PF₆] medium was recycled 10 times without substantial diminution in activity.CONCLUSION: The ionic liquid [i-C₄mim][PF₆] has good biocompatibility, and can be used widely as green media in various biocatalysis reactions to improve the activity and stability of enzymes. Besides hydrophobicity and nucleophilicity, the spatial configuration of ionic liquids is also considered a key factor effecting the behaviour of the enzyme in ionic liquids.
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