Developmental regulation of an instability element from the Drosophila fushi tarazu mRNA
2001
Fontes, A.M. | Riedl, A. | Jacobs-Lorena, M.
The Drosophila fushi tarazu (ftz) mRNA is one of the shortest-lived metazoan mRNAs, and its instability is crucial for proper development of the embryo. Previously, we identified two cis-acting elements that are required for ftz mRNA degradation, one within the 5' one-third and another in the 3'UTR of the message. Here we focus on the 3'UTR element termed FIE3 (ftz instability element in the 3'UTR). To investigate the developmental regulation of the FIE3-dependent degrading activity we measured the abundance of an FIE3-containing mRNA in ovaries, unfertilized eggs, and different larval and adult tissues. We found that FIE3-degrading activity is present at all developmental stages and tissues examined, except in the ovary. Activation of the FIE3-dependent mRNA decay is independent of fertilization because it could be triggered by egg activation. Finally, we provide evidence that mutation of conserved elements within FIE3 had no effect on mRNA instability.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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