Determination of irradiation histories of raw beef livers using liquid chromatography–tandem mass spectrometry of 5,6-dihydrothymidine
2017
Fukui, Naoki | Takatori, Satoshi | Kitagawa, Yoko | Okihashi, Masahiro | Ishikawa, Etsuko | Fujiyama, Takatomo | Kajimura, Keiji | Furuta, Masakazu | Obana, Hirotaka
A method for detecting irradiation histories of raw beef livers was developed by measuring 5,6-dihydrothymidine (DHdThd) using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Liver DNA was extracted using phenol–chloroform extraction followed by precipitation in 50% ethanol. DNA was then enzymatically digested and nucleosides were purified using an OASIS MCX column. DHdThd and thymidine (dThd) contents of resulting test solutions were analyzed using LC–MS/MS. DHdThd was detected specifically after γ-irradiation. Concentration ratios of DHdThd to dThd in the test solutions increased dose-dependently after irradiation at 1.0–11.3kGy, which included the practical dose for sterilization of 2–7kGy. Dose–response curves from beef livers of individual animals almost overlapped. Thus, this method is a candidate for the detection of irradiation histories of foods from which DNA can be extracted.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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