Effect of biosolids characteristics on retention and release behavior of azithromycin and ciprofloxacin

Azithromycin (AZ) and ciprofloxacin (CIP) are commonly prescribed antibiotics frequently detected in municipal biosolids and identified by the USEPA as contaminants of emerging concern. The land application of municipal biosolids is an agronomically beneficial practice but is also a potential pathway of CIP and AZ release into the environment. Understanding retention-release behavior is crucial for assessing the environmental fate of and risks from land-applied biosolids-borne target antibiotics. Here, we used batch equilibrations to assess retention and release of environmentally relevant concentrations of CIP and AZ in ten different biosolids. The biosolids included Class A and Class B materials with a range of physiochemical characteristics (e.g. pH, cation exchange capacity (CEC), organic matter content (OM), and iron (Fe) and aluminum (Al)) expected to influence retention and release of AZ and CIP. Retention was linear (R² > 0.99 for AZ and >0.96 for CIP) and sorption coefficients (Kd) ranged from 52 to 370 L kg⁻¹ for AZ and 430–2300 L kg⁻¹ for CIP. Desorption also varied but was highly hysteretic, with hysteresis coefficients (H) ranging 0.01 to 0.15 for AZ and ≤0.01 for CIP, suggesting limited bioaccessibility. The penalized and shrinkage method least absolute shrinkage and selection operator (LASSO) was used to produce models describing AZ and CIP sorption behavior based on any given biosolids physiochemical characteristics. Multiple linear regression analysis linked AZ sorption behavior to total Fe content, likely due to a predisposition of AZ to participate in reactions with in situ Fe species. CIP sorption behavior was linked to oxalate extractable Al and total phosphorus (P) content, suggesting CIP bonding with amorphous forms of Al and a potential relationship between CIP sorption to biosolids and biosolids production processes, as manifested by correlation of CIP sorption with total P content.