Convenient synthesis of nucleoside 5′-triphosphates for RNA transcription
2011
Caton-Williams, Julianne | Lin, Lina | Smith, Matthew | Huang, Zhen
By generating a selective phosphitylating reagent in situ, nucleoside 5′-triphosphates can be conveniently synthesized in one pot. This novel strategy without nucleoside protection has been developed to largely simplify synthesis of the nucleoside triphosphates. This demonstrated principle can be applied to the 5′-triphosphate synthesis of both native and modified nucleosides.
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