Perturbations in nucleosome structure from heavy metal association
2010
Mohideen, Kareem | Muhammad, Reyhan | Davey, Curt A.
Heavy metals have the potential to engage in strong bonding interactions and can thus function in essential as well as toxic or therapeutic capacities. We conducted crystallographic analyses of heavy cation binding to the nucleosome core particle and found that Co²⁺ and Ni²⁺ preferentially associate with the DNA major groove, in a sequence- and conformation-dependent manner. Conversely, Rb⁺ and Cs⁺ are found to bind only opportunistically to minor groove elements of the DNA, in particular at narrow AT dinucleotide sites. Furthermore, relative to Mn²⁺ the aggressive coordination of Co²⁺ and Ni²⁺ to guanine bases is observed to induce a shift in histone-DNA register around the nucleosome center by stabilizing DNA stretching over one region accompanied by expulsion of two bases at an opposing location. These 'softer' transition metals also associate with multiple histone protein sites, including inter-nucleosomal cross-linking, and display a proclivity for coordination to histidine. Sustained binding and the ability to induce structural perturbations at specific locations in the nucleosome may contribute to genetic and epigenetic mechanisms of carcinogenesis mediated by Co²⁺ and Ni²⁺.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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