Genetic mapping and development of near-isogenic lines for genes governing a liguleless phenotype in tetraploid wheat
2015
Amagai, Y. | Watanabe, N. | Kuboyama, T.
The liguleless phenotype in wheat lacks ligules and auricles on all plant leaves. Two duplicate dominant genes (Lg ₁ and Lg ₃) principally control the presence of ligules in tetraploid wheat. Although the Lg ₃ gene was tentatively located in chromosome 2A, a genetic stock of Triticum durum Desf. (2n = 4x = 28, BBAA genome) having genotype lg ₁ lg ₁ Lg ₃ Lg ₃ has not been available. We speculated that a wild emmer (T. dicoccoides Körn., 2n = 4x = 28, BBAA genome) could be a possible source of the Lg ₃ gene. In the present study near-isogenic lines (NIL’s) ANW 12E for genotype lg ₁ lg ₁ Lg ₃ Lg ₃ and ANW 12F for genotype Lg ₁ Lg ₁ lg ₃ lg ₃ were developed from a cross between ANW 12A (lg ₁ lg ₁ lg ₃ lg ₃), a liguleless NIL of T. durum LD222, and LDN(DIC 2A) (Lg ₁ Lg ₁ Lg ₃ Lg ₃) through backcrossing to ANW 12A. LDN(DIC 2A) is a chromosome substitution line in which a pair of T. durum cv. Langdon (LDN) 2A chromosomes is replaced with a pair of chromosomes 2A from T. dicoccoides (DIC) line Israel A. Molecular mapping using the F₂ of liguleless durum PI 370751/ANW 12F revealed that the Lg ₁ gene was 17.5 cM distal to Xgwm47 in chromosome 2BL. In the F₂ of PI 370751/ANW 12E the Lg ₃ gene was located 18.6 cM distal to Xgwm312 in chromosome 2AL. These results confirmed that the Lg ₃ locus was present in chromosome 2A, and dominant alleles at the homoeologous Lg loci determine the presence of ligules in polyploid wheat.
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