Detoxification of commercial United Kingdom rapeseed meal by glucosinolate hydrolysis with exogenous myrosinase and the extractability of the aglucones by aqueous industrial methylated spirits
1989
Finnigan, T.J.A. | Lewis, M.J. | Dietz, M.
The aims of this study were to investigate the detoxification of United Kingdom commercial rapeseed meal by alcoholic extraction of aglucones released after treatment with mustard seed myrosinase. Aglucones released from the commercial rapeseed meal were compared with those resulting from autolysis of laboratory-defatted rapeseed. 3-Butenyl isothiocyanate, 5-vinyl-oxazolidene-2-thione (VOT) and 1-cyano-2-hydroxy-3-butene were detected in commercial rapeseed meal after glucosinolate hydrolysis. In comparison, autolysis of rapeseed at 5 degrees C gave VOT, 1-cyano-2-hydroxy-3-butene and epithionitriles, but at 60 degrees C epithionitrile release was significantly reduced. In addition, 1-cyano-3,4-epithiobutane was detected in the autolysed samples. Aglucones released at 40 degrees C from commercial rapeseed meal by mustard seed myrosinase were 85% extractable in 60-90% (v/v) aqueous industrial methylated spirits. Extraction was significantly higher than previously reported for the intact glucosinolate. Double extraction of the myrosinase-treated meal with 80% (v/v) aqueous industrial methylated spirits gave a meal with no detectable intact glucosinolate or aglucone content.
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