Effects of adrenergic agonists on glycogenolysis in primary cultures of glycogen body cells and telencephalon astrocytes of the chick
2001
Lee, K. | Makino, S. | Imagawa, T. | Kim, M. P (Maksim Pavlovich) | Uehara, M.
The glycogen body (GB) is at the dorsal area of the lumbosacral spinal cord in birds and is composed of uniform cells that are characterized by high-glycogen storage. Previous morphological and embryological examinations suggest that the GB is derived from the neuroepithelium and contains many blood vessels and a few nerve fibers. However, the function of the GB and role of the glycogen are unknown. Mammalian astrocytes are major sites for glycogen stores in the central nervous system. The metabolic features of astrocytes have been defined by using cultured cells. As a first step toward investigating the function of GB, we established primary culture of chicken GB cells and telencephalon astrocytes. The cultured GB cells maintained high glycogen content and glial fibrillary acidic protein (GFAP) in the cytoplasm. The glycogen content of GB cells significantly increased with the glucose concentration in the medium. The effects of adrenergic agonists on glycogenolysis were different between GB cells and telencephalon astrocytes. The telencephalon astrocytes shared similar characteristics of glycogenolysis with mouse astrocytes, which are mainly affected by beta adrenergic receptor. Although GB cells were affected by noradrenalin (both alpha and beta adrenergic agonist), they were not affected by beta adrenergic agonist. These results showed that cultured GB cells were considered as one lineage of astrocytes because of their reactivity to antibody against GFAP; however, the metabolic features of GB cells were different from those of telencephalon astrocytes.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل National Agricultural Library