Taxonomic significance of the lipopolysaccharide composition of the three biovars of Agrobacterium tumefaciens
1993
Weibgen, U. | Russa, R. | Yokota, A. | Mayer, H.
The sugar and fatty acid composition of 9 strains belonging to the 3 biovars of Agrobacterium tumefaciens and to Agrobacterium rubi was investigated. All strains showed a complex composition of the sugar moieties which comprised mostly 6-deoxyhexoses, hexoses and acidic sugars, such as GalA, 3-deoxy-lyxo-heptulosaric acid and Kdo. More striking however, was the different pattern in DOC-PAGE indicating that biovar 3 strains (Agrobacterium vitis), as well as Agrobacterium rubi contained an R-type LPS (or an R-type with only a very truncated O-chain). In contrast, strains of biovars 1 and 2 showed-very long O-chains comprising more than 20 O-repeating units. The fatty acid profiles of the lipid A moieties of the LPS of biovars 1, 2 and 3 were characterized by the predominance of hydroxylated fatty acids which comprised 2-5 different 3-OH-fatty acids, as well as (n-2) hydroxylated long chain fatty acids, such as 27-OH-28:O. Biovar 2 strains were characterized by a high content of the rare iso-branched 3-OH-15:O, which was lacking in the other biovars and may serve as a characteristic chemotaxonomic marker. The presence of the rare 3-OH-18:O and 3-OH-18:1 in 7 out of 9, or 5 out of 9 strains, respectively, was conspicuous.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل National Agricultural Library