Impairment of testicular development in rats exposed to acephate during maternal gestation and lactation

Sampaio, Carolina Ferreira; Prates, Kelly Valério; Siervo, Gláucia Eloisa Munhoz Lion; Mathias, Paulo Cézar de Freitas; Fernandes, Glaura Scantamburlo Alves

Impairment of testicular development in rats exposed to acephate during maternal gestation and lactation

2020

Sampaio, Carolina Ferreira | Prates, Kelly Valério | Siervo, Gláucia Eloisa Munhoz Lion | Mathias, Paulo Cézar de Freitas | Fernandes, Glaura Scantamburlo Alves

Acephate is an organophosphate insecticide that disrupts the endocrine system and impairs the male reproductive system. Thus, the aim of the present study was to evaluate whether exposure to acephate during maternal gestation and lactation histologically damages the testes of male Wistar rats in adulthood. For this study, adult Wistar rats were divided into the following groups: ACE-mother, (2.5 mg/kg/bw, gestational day (GD) 7 to postnatal day (PND) 21) and oil-mother (corn oil (control), GD 7 to PND 21). The male offspring (PND 90) were euthanized, and the prostates and testes were collected and weighed. The testes were utilized for histopathological analyses and to determine the sperm count. A spermatogenesis kinetic analysis revealed an increased number of seminiferous tubules in stages I–VI in the ACE-mother group. Additionally, we observed a decrease in the epithelium and the diameters of the evaluated seminiferous tubules and in the number of Sertoli cells in the group exposed to acephate. The sperm count analysis showed no difference between the groups. We conclude that maternal exposure to the pesticide acephate did not affect testicular function, but led to the impairment of testicular development and morphology of the tissue in adulthood.

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