Elemental Analysis of Different Cultivar of Senna (Cassia angustifolia) using Microwave Digestion and Flame Atomic Absorption Spectroscopy
2017
Basak, B. B. | Nagaraja Reddy, R.
Senna (Cassia angustifolia Vahl.) is known worldwide for its laxative properties. Essential element content in senna leaf and pod is important as they preferred as an ingredient of herbal tea, functional food and dietary supplements. So, elemental (Ca, Mg, K, Na, Fe, Mn, Zn and Cu) content in the leaf and pod samples of four different cultivar of senna were determined by Flame Atomic Absorption Spectroscopy after digestion in the closed vessel environment through a microwave digestion system. Powdered leaf and pod samples were digested with high-purity concentrated nitric acid (HNO₃). The overall reproducibility of the method obtained from spiking experiment was within the range and recovery data indicated accurate and precise. The elemental content in the leaf and pod sample followed the order Ca > K > Mg > Na > Fe > Zn > Mn > Cu. While elemental content in pod samples were found lower as compared to leaf samples except potassium. Calcium content was found higher than other elements and level of Copper was the least among all elements in the leaf and pod samples. Significant differences were observed for elemental content among the cultivars. The cultivar KKM-1 recorded higher elemental content compared to other cultivars. This basic information generated from the study can be used for the varietal development of senna with improved nutritional quality apart from its medicinal properties.
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