Gliadin nanoparticles stabilized by a combination of thermally denatured ovalbumin with gemini dodecyl O-glucoside: The modulating effect of cosurfactant
2017
Feng, Jin | Wu, Shanshan | Wang, Hua | Liu, Songbai
In this work, gliadin nanoparticles were fabricated by liquid antisolvent precipitation (LAS) method using a synergistic combination of thermally denatured ovalbumin (OVA) with cosurfactant gemini dodecyl O-glucoside (Gemini-C12) as stabilizers. The interaction between OVA and Gemini-C12 was characterized by surface tension, fluorescence spectroscopy, isothermal titration calorimetry, and circular dichroism analysis, respectively. The relationship between the properties of gliadin nanoparticles and Gemini-C12 concentration was interpreted on the basis of OVA-Gemini-C12 interaction. When the concentration below critical micelle concentration (CMC), Gemini-C12 could decrease the interfacial tension and enhance the adsorption strength of OVA on particle surface, thereby increasing the nucleation rate and giving gliadin nanoparticles with smaller size. Besides, the monomeric cosurfactant could plug the vacancies left by OVA at solid-liquid interface and therefore prohibit the aggregation and agglomeration of gliadin nanoparticles during storage and freeze-drying. Above CMC, free micelles were formed in the bulk and the surfactant molecules tended to reside in micelles rather than be adsorbed at the solid-liquid interface, which compromised the protective effect of cosurfactant and resulted in gliadin nanoparticles with larger size and lower storage stability and redispersibility.
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