Comparison Between New Wild Plant Trifolium repens and Vicia faba on their Sensitivity in Detecting the Genotoxic Potential of Heavy Metal Solutions and Heavy Metal-Contaminated Soils
2009
Manier, Nicolas | Deram, Annabelle | Le Curieux, Frank | Marzin, Daniel
The DNA damage and cytotoxicity induced by CdCl₂ solutions and soils anthropogenically contaminated with heavy metals were studied using the micronucleus (MN) test. Vicia faba, a plant model widely used in liquid exposure assays, was adapted for direct exposure to a solid phase. In addition, the MN assay was adapted to a new wild plant system, the white clover (Trifolium repens). The results obtained after exposure to CdCl₂ solutions confirmed that V. faba root cells were a sensitive model and revealed that T. repens root cells were not appropriate for the detection of micronuclei (although they were highly sensitive to the cytotoxic effect of CdCl₂). Concerning the results observed after direct exposure to contaminated soils (solid-phase exposure), the MN frequency scores in V. faba root cells were increased in a statistically significant and dose-related manner compared to the control plants. Regarding T. repens root cells, this solid phase exposure confirmed that this model is not appropriate for use in the micronucleus assay.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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