Pendant small functional groups on poly(ϵ-caprolactone) substrate modulate adhesion, proliferation and differentiation of human mesenchymal stem cells
2015
Chen, Min | Zhang, Yi | Zhou, Yan | Zhang, Yan | Lang, Meidong | Ye, Zhaoyang | Tan, Wen-Song
Probing stem cell–biomaterial interactions is of great significance in both gaining profound understanding of stem cell biology and advancing tissue regeneration. In the present work, we developed a series of poly(ϵ-caprolactone) (PCL) films bearing distinct pendant small functional groups to study the effects of biomaterial substrate chemistry on stem cell behaviors. PCL films, bearing hydroxyl (OH), methyl (CH3), carboxyl (COOH) and amino (NH2), demonstrated varied surface properties, such as wettability, serum protein adsorption and surface topographical feature. In comparison with pristine PCL film, the adhesion of hMSCs on PCL–COOH, PCL–OH and PCLCO films was significantly promoted and cells slightly outgrew on PCL–NH2 and PCL–COOH films. Most importantly, the tri-lineage differentiation of hMSCs varied on this series of PCL films, with the best osteogenesis achieved on PCL–NH2 film, PCL and PCL–CH3 films supporting the superior adipogenic differentiation and PCL–CH3 film being the most favorable one for chondrogenesis. This study highlights the critical roles of surface chemistry in modulating the fates of MSCs and potentially provides a practical guidance in developing instructive tissue engineering scaffolds.
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