The alpha 1-tubulin gene of Arabidopsis thaliana: primary structure and preferential expression in flowers
1988
Ludwig, S.R. | Oppenheimer, D.G. | Silflow, C.D. | Snustad, D.P.
The primary structure of the alpha 1 tubulin gene of Arabidopsis thaliana was determined and the 5' and 3' ends of its transcript were identified by S1 nuclease mapping experiments. The information obtained was used to (i) predict the amino acid sequence of the alpha 1-tubulin, (ii) deduce the positions of introns within the alpha 1-tubulin gene, and (iii) construct 3' noncoding gene-specific hybridization probes with which to study the pattern of alpha 1-tubulin transcript accumulation in different tissues and at different stages of development. The predicted amino acid sequence of the alpha 1-tubulin has 92% identity with the predicted product of the previously characterized A. thaliana alpha 3-tubulin gene. The coding sequence of the alpha 1-tubulin gene is interrupted by four introns located at positions identical to those of the four introns in the alpha 3 gene. RNA blot hybridization studies carried out with an alpha 1-tubulin gene-specific probe showed that the alpha 1 gene transcript accumulates primarily in flowers, with little transcript present in RNA isolated from roots or leaves. In order to investigate the pattern of alpha-tubulin gene expression in developing flowers, RNA was isolated from flowers at five different stages of development: flower buds, unopened flowers with pollen, open flowers, flowers with elongating carpels, and green seed pods. RNA blot hybridizations performed with 3' noncoding gene-specific probes showed that the alpha 3 tubulin gene transcript is present in flowers at all stages of development, whereas the alpha 1-tubulin gene transcript could only be detected in RNA from unopened flowers with pollen, open flowers, and flowers with elongating carpels.
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