Uniquely regulated proteinase inhibitor I gene in a wild tomato species: Inhibitor I family gene is wound-inducible in leaves and developmentally regulated in fruit
1991
Wingate, V.P.M. | Ryan, C.A.
A uniquely regulated proteinase inhibitor I gene was isolated from the wild tomato species Lycopersicon peruvianum (L.) Mill. (LA107) and characterized. The inhibitor gene is wound-inducible in leaves and is expressed in unripe fruit during development. The gene (lambda clone 1) is present on a 15.5 kilobase pair Sal1-SalI genomic DNA fragment. Southern blot analysis of L. peruvianum genomic DNA shows only one strongly hybridizing DNA fragment to probes derived from X clone 1. S1 nuclease protection experiments and Northern analyses confirm that this gene is both wound-inducible in leaves and developmentally regulated in young unripe fruit. These observations are supported by comparisons of the 5'-flanking DNA sequences of the L. peruvianum inhibitor I gene with known elicitor responsive cis-acting sequences. The transcriptional regulation of the lambda clone 1 inhibitor I gene in leaves of wounded plants and in developing unripe fruit indicates that the gene contains unique complex regulating elements. These elements respond to both environmental and developmental tissue-specific signals that can regulate proteinase inhibitor synthesis to protect the tissues of this wild species of tomato against predators and pathogens.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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