Rheology and microstructure of cross-linked waxy maize starch/whey protein suspensions
2009
Vu Dang, H. | Loisel, C. | Desrumaux, A. | Doublier, J.L.
The objective of the present work was to investigate the effect of the heating process on the structural and rheological properties of whey protein isolate/cross-linked waxy maize starch (WPI/CWMS) blends depending upon the concentration and the starch/whey protein ratio. Starch concentration ranged from 3 to 4% (w/w) and the protein content was of 0.5, 1 and 1.5% (w/w). The blend (pH 7, 100mM ionic strength) was heated using a jacketed vessel at two pasting temperatures: 90 and 110^oC. The particle size distribution of the WPI suspension (1.5%) displayed three distinct classes of aggregates (0.3, 65 and 220@mm), whereas the size of swollen starch granules varied from 48 to 56@mm according to the pasting temperature. When the two components were mixed together, the peak attributed to swollen starch granules was attenuated and broadened towards higher values (up to 88@mm) due to protein aggregates (260-410@mm). This effect was more pronounced as the protein concentration increased. When compared to starch alone, the rheology of the mixed system was dramatically modified for the flow behaviour as well as for the viscoelastic properties which changed from a solid-like (3-4% starch) to a liquid-like behaviour (3-4% starch/1.5% protein). Microscopic observations showed aggregated proteins located in the continuous phase and swollen starch granules as the dispersed phase. Protein aggregates were of different sizes, part of them appeared adsorbed onto swollen starch granules while another part was unevenly distributed in the continuous phase, yielding discontinuous network which could explain the peculiar viscoelastic behaviour of such suspensions.
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