Mapping of major and modifying genes for high oleic acid content in safflower
2012
Hamdan, Yamen A. S. | García-Moreno, María J. | Fernández-Martínez, José M. | Velasco, Leonardo | Pérez-Vich, Begoña
Oils with high oleic acid content are in great demand because they have optimal properties for food and non-food uses. Two different levels of high oleic acid content (>75 and >84%) have been reported in safflower (Carthamus tinctorius L.). The trait is mainly controlled by partially recessive alleles at a major gene Ol, but the highest levels have been attributed to modifying genes. The objectives of this research were to map the Ol locus and modifying genes involved in oleic acid content of safflower seeds and to determine the nature of Ol through a candidate gene approach. Two F2 mapping populations from the nuclear male-sterile line CL-1 and the high oleic acid lines CR-6 (>75% oleic acid) and CR-9 (>84%) were developed and phenotyped for oleic acid content at the F2 and F3 seed level. A genetic linkage map comprising 15 linkage groups and 116 random amplified polymorphic DNA, simple sequence repeat (SSR), and sequence-characterized amplified regions marker loci was constructed for the CL-1 × CR-9 population. The Ol gene was mapped to linkage group (LG) T3 tightly linked to the SSR marker ct365, which was confirmed in the CL-1 × CR-6 population. Additionally, a quantitative trait locus with a minor effect on increasing oleic acid content was identified on LG T2. The candidate gene approach indicated that an oleoyl-phosphatidylcholine desaturase FAD2-1 locus underlies the Ol gene. Both the genetic information and the markers developed in this research will contribute to marker-assisted selection for high oleic acid content in safflower.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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