ROS-induced PCD affects the viability of seeds with different moisture content after cryopreservation
2022
Ren, Ruifen | Zhou, Hao | Zhang, Lingling | Jiang, Xueru | Zhang, Min | Liu, Yan
Moisture content is one of the important factors affected the cryopreservation of seeds, but it is not completely clear how moisture content affects the viability of seeds after cryopreservation. In this study, compared the changes of programmed cell death (PCD) and reactive oxygen species (ROS) of wild Paeonia emodi seeds with different moisture content before and after cryopreservation, and their relationship with viability, to investigate the role of ROS- induced PCD in seed viability after cryopreservation with different moisture content. The results showed that: seed viability significantly decreased and the ROS content increased with the increase of moisture content after cryopreservation. With the appearance of typical morphological characteristics of PCD such as cytoplasmic wall separation, cell membrane rupture and nuclear shrinkage; the activity of caspase-3-like protease was significantly increased and mitochondrial membrane potential decreased, which involved in the occurrence of PCD. The seeds were treated with exogenous hydrogen peroxide (H₂O₂) after cryopreserved, which significantly promoted the occurrence of PCD and significantly reduced the viability of seeds; however, the incubation of exogenous ROS scavenger ascorbic acid (AsA) had the opposite effects. These results indicated that ROS induced PCD affected the seed viability after cryopreservation. The ROS concentration of seeds with different moisture content was different after cryopreservation, and the occurrence degree of PCD was different, which leaded to the difference of viability after cryopreservation.
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