Effects of semen extender and semen processing on motility and viability of frozen-thawed dog spermatozoa
2006
Schafer-Somi, S. | Kluger, S. | Knapp, E. | Klein, D. | Aurich, C.
The aims of the present study were, to assess the effects of semen centrifugation, two different diluents and two different freezing methods on post-thaw semen quality in canine semen, and to elucidate the interdependence of these parameters. For this purpose, the sperm-rich fractions of ejaculates from 12 healthy male beagles were divided into four aliquots. Two aliquots were centrifuged and resuspended with two TRIS-egg yolk based extenders: with Uppsala [1] and Gill extender (Gill). The diluents differed in the concentration of glycerol and in the admixture of Equex STM paste (Nova Chemical Sales Inc., Scituate, MA, USA). Diluted semen was frozen either in a styrofoam box or with a computerized freezing machine and an optimized freezing curve (IceCube 1810; Sy-Lab, Purkersdorf, A). The change in temperature inside the straws was measured during the freezing procedure.
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