The effect of ATP on cucumber (Cucumis sativus L.) regeneration from nodal explants: association with α-tocopherol, H2O2 and size of culture vessel
2005
Matakiadis, T. | Kintzios, S.
We investigated the effect of adenosine 5'-triphosphate (ATP), α-tocopherol and H202 on the micropropagation of cucumber (Cucumis sativus L.) from nodal explants. The effect of the size of the liquid culture vessel (250-ml flask vs. 2.5-l airlift bioreactor) was also evaluated. The addition of ATP alone caused a significant increase in the number of branches (internodes) and in internodal length, but also a reduction of leaf number, compared to control cultures. It also increased significantly the accumulation of NO3- and K+ . The application of ATP + α-tocopherol was associated with a significant increase in bud, internodal, leaf and petiole number, internodal, petiole and root length, as well as plant fresh weight, but reduced PO43- and Ca2+ accumulation. The combined application of ATP + α-tocopherol + H2O2 was associated with maximum petiole length and increased plant fresh weight but reduced Ca2+ accumulation. Compared to all other treatments, application of ATP + α-tocopherol in bioreactor-incubated cultures produced significantly larger plants, with an increased bud number, internode lenght and soluble carbohydrate concentration, but also with a reduced fresh weight, root length and reduced NO3- and PO43- and Ca2+ concentrations. These effects were associated with changes in the redox status of the regenerants, as well as dehydrogenase and peroxidase activities. The perspective for an application of ATP and antioxidants as novel, cost-efficient growth regulators in the micropropagation of this commercially important vegetable species is discussed.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
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