Dietary Tributyrin Attenuates Intestinal Inflammation, Enhances Mitochondrial Function, and Induces Mitophagy in Piglets Challenged with Diquat
2019
Wang, Chunchun | Cao, Shuting | Zhang, Qianhui | Shen, Zhuojun | Feng, Jie | Hong, Qihua | Lu, Jianjun | Xie, Fei | Peng, Yan | Hu, Caihong
The study evaluated the effects of butyric acid, in the form of tributyrin on the oxidative stress, inflammation, and mitochondrial function in diquat-challenged pigs. Twenty-four weaned pigs were allocated to four treatments in a 2 × 2 factorial arrangement with the main effects of tributyrin supplementation and diquat challenge. The results showed that supplemental tributyrin increased (P < 0.05) average daily gain and average daily feed intake of diquat-challenged pigs. Tributyrin elevated (P < 0.05) the activities of total antioxidant capacity and superoxide dismutase, reduced (P < 0.05) malondialdehyde content, and increased (P < 0.05) mRNA levels of copper and zinc superoxide dismutase and manganese-containing superoxide dismutase of diquat-challenged pigs. Tributyrin relieved (P < 0.05) intestinal inflammation reflected by decreased mRNA abundances of tumor necrosis factor-α, interferon-γ, and interleukin-6 in the intestine. Tributyrin reduced (P < 0.05) serum diamine oxidase activity and d-lactate content, increased (P < 0.05) transepithelial electrical resistance, decreased paracellular flux of dextran (4 kDa), and prevented the diquat-induced decrease (P < 0.05) in the expressions of claudin-1, occludin, and zonula occludens-1. Tributyrin alleviated (P < 0.05) diquat-induced mitochondrial dysfunction shown by lowered reactive oxygen species, increased mitochondrial membrane potential, and increased adenosine triphosphate content. Furthermore, tributyrin increased (P < 0.05) expressions of mitophagy proteins (PTEN-induced putative kinase 1 and Parkin), and ratio of light chain 3-II to light chain 3-I in intestine. Collectively, tributyrin attenuated oxidative stress and intestinal inflammation, improved mitochondrial function, and induced mitophagy in diquat-challenged pigs.
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