Microplastics: What happens in the human digestive tract? First evidences in adults using in vitro gut models
2023
Fournier, Elora | Lévêque, Mathilde | Ruiz, Philippe | Ratel, Jeremy | Durif, Claude | Chalancon, Sandrine | Amiard, Frederic | Edely, Mathieu | Bezirard, Valerie | Gaultier, Eric | Lamas, Bruno | Houdeau, Eric | Lagarde, Fabienne | Engel, Erwan | Etienne-Mesmin, Lucie | Blanquet-Diot, Stéphanie | Mercier-Bonin, Muriel
Microplastics (MPs) are ubiquitous in the environment and humans are inevitably exposed to them. However, the effects of MPs in the human digestive environment are largely unknown. The aim of our study was to investigate the impact of repeated exposure to polyethylene (PE) MPs on the human gut microbiota and intestinal barrier using, under adult conditions, the Mucosal Artificial Colon (M-ARCOL) model, coupled with a co-culture of intestinal epithelial and mucus-secreting cells. The composition of the luminal and mucosal gut microbiota was determined by 16S metabarcoding and microbial activities were characterized by gas, short chain fatty acid, volatolomic and AhR activity analyses. Gut barrier integrity was assessed via intestinal permeability, inflammation and mucin synthesis. First, exposure to PE MPs induced donor-dependent effects. Second, an increase in abundances of potentially harmful pathobionts, Desulfovibrionaceae and Enterobacteriaceae, and a decrease in beneficial bacteria such as Christensenellaceae and Akkermansiaceae were observed. These bacterial shifts were associated with changes in volatile organic compounds profiles, notably characterized by increased indole 3-methyl- production. Finally, no significant impact of PE MPs mediated by changes in gut microbial metabolites was reported on the intestinal barrier. Given these adverse effects of repeated ingestion of PE MPs on the human gut microbiota, studying at-risk populations like infants would be a valuable advance.
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