Speciation of selenium in diet supplements by HPLC-MS/MS methods
2007
Gosetti, F. | Frascarolo, P. | Polati, S. | Medana, C. | Gianotti, V. | Palma, P. | Aigotti, R. | Baiocchi, C. | Gennaro, M.C.
HPLC-MS/MS methods are developed to study the speciation of selenium in food supplements. The species most commonly present in supplements are considered: seleno-l-methionine (C5H11O2NSe), l-selenocystine (C6H12O4N2Se2), phenyl-l-selenocysteine (C9H11O2NSe), methyl-seleno-l-cysteine (C4H9O2NSe), methaneseleninic acid (CH3SeO2H), selenate (SeO4(2-), selenocyanate (SeCN-) and selenite (SeO3(2-). The chromatographic analysis is performed on a Luna C18 stationary phase: for the separation of the organic species the mobile phase is a mixture of methanol and water both 0.05% in trifluoroacetic acid, while for the separation of inorganic species the mobile phase is a mixture of methanol and tetrabutylammonium hydroxide 1.0 mM aqueous solution. Gradient elution conditions are used in both the separations. Two mass spectrometry analyzers are employed for detection, namely an ion trap for determination of the aminoacidic species and a triple quadrupole for the inorganic ones.
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