The purification of pectin from commercial fruit flours results in a jaboticaba fraction that inhibits galectin-3 and colon cancer cell growth
2020
do Nascimento, Raissa Sansoni | Pedrosa, Lucas de Freitas | Diethelm, Luiza Tamie Hirata | Souza, Thales | Shiga, Tania M. | Fabi, João Paulo
Fruits are a prime source of nutrients, bioactive compounds, and dietary fibers. Some products available on the Brazilian market use fruit by-products and claim to have useful effects on human health due to their dietary fiber content. The study aimed to extract and purify the total (28–47 w/w yield) and soluble dietary fiber (4–7 w/w yield) from jaboticaba, papaya, and plum commercial flours sold in Brazil and to study the in vitro biological effects of the fractions. The purified water-soluble fractions consisted mainly of pectin-derived oligosaccharides (5–15 KDa molecular weight) with a negligible content of polyphenols, protein, ashes, and starch. Jaboticaba sample was 95% galacturonic acid while plum and papaya samples were 40% galacturonic acid and 40% galactose (mol%), approximately. The samples were tested for recombinant human galectin-3 inhibition and changes in the cell viability of human colorectal cancer cells. Only the jaboticaba sample inhibited galectin-3 and decreased HCT116 cell viability after 48 h of treatment (p ≤ 0.01) while the plum sample decreased the cell viability after 24 h treatment (p ≤ 0.05). The results obtained in this study demonstrate the relationship between the structure of the soluble fibers extracted from jaboticaba flour and the possible beneficial effects of their consumption.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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