Expression and characterization of a novel lytic polysaccharide monooxygenase, PdLPMO9A, from the edible fungus Pleurotus djamor and its synergistic interactions with cellulase in corn straw biomass saccharification
2022
Li, Yanli | Li, Tongbing | Guo, Juntong | Wang, Gang | Chen, Guang
Lytic polysaccharide monooxygenases play a unique role in biomass saccharification. A novel gene, PdLPMO9A, from Pleurotus djamor, was reported to be upregulated during the degradation of corn straw in our previous research. However, very little information is available on PdLPMO9A. Therefore, PdLPMO9A was heterologously expressed in Pichia pastoris, and biophysical characterisitics of the recombinant protein PdLPMO9A were investigated; it was shown to have superior thermostability and pH stability. PdLPMO9A markedly improved the cellulase-mediated saccharification of corn straw, when the dosage of PdLPMO9A was 0.66 mg/g corn straw and hydrolysis time was 48 h. When CuSO₄ was added at a concentration of 0.1 mM, glucose yield rose by a further 28.16%. In light of these findings, it was concluded that PdLPMO9A has the potential to function as an essential component of a cellulase cocktail capable of ensuring the saccharification of corn straw biomass.
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