Ethanolic extraction of commercial rapeseed meal in the production of protein concentrate of reduced glucosinolate content
1988
Finnigan, T.J.A. | Lewis, M.J.
The aim of this work was to investigate suitable extraction procedures for the production of a protein concentrate of significantly reduced glucosinolate content from commercial rapeseed meal. The level is a factor in deciding its suitability for either animal or human food. Ethanolic extraction was studied as a function of time, solvent to meal ratio and solvent composition, to minimise concomitant losses of solids and nitrogen. Rapeseed meal was extracted over the range of 0 to 100% (v/v) aqueous alcohol. A concentration of 80% was found to give the best compromise between glucosinolate extraction and other component loss, but glucosinolate extraction was only about 30%. Increase of solvent polarity improved glucosinolate extractability, but extraction yields greater than 70% were not found. Subsequent extraction of the pretreated meal with water at pH 11.0 gave extracts of lower glucosinolate concentration than those made from non-pretreated meal, but residual concentrations of the glucosinolate were unacceptable. Additional hydrolysis of the glucosinolates by exogenous myrosinase is proposed to improve extractability.
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