Response of Lactobacillus casei BL23 to phenolic compounds
2011
RivasâSendra, A. | Landete, J.M. | Alcántara, C. | Zúñiga, M.
Aims: To determine the inhibitory effect of phenolic compounds on Lactobacillus casei BL23, the role of two component signal transduction systems (TCS) and the response of Lact. casei BL23 to pâcoumaric acid. Methods and Results: Growth of Lact. casei BL23 and 17 derivative strains defective in each TCS harboured by this strain in the presence of pâcoumaric acid, ferulic acid, caffeic acid or methyl gallate was monitored. Furthermore, changes in the protein content of Lact. casei BL23 when exposed to pâcoumaric acid were evaluated by 2DâSDSâPAGE. Eleven proteins differentially expressed in the presence of pâcoumaric acid were detected. Six of them could be identified: ClpP and HtrA, involved in protein turnover and folding, acetylâCoA carboxylase, involved in lipid metabolism, and an arginylâtRNA synthetase were more abundant, whereas PurL and PurN, involved in purine biosynthesis, were less abundant. Conclusions: No significant differences were observed between the parental strain and the TCSâdefective mutants. pâCoumaric acid elicited a response against membrane and cytoplasmic damages. Significance and Impact of the Study: The inhibitory effect of phenolic compounds on Lact. casei BL23 has been determined. For the first time, cytoplasmic proteins presumably involved in the response of Lact. casei BL23 against pâcoumaric acid have been identified.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
تم تزويد هذا السجل من قبل National Agricultural Library