Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish
2008
Tsuchiya, C. | Sakata, T. | Sugita, H.
This study was conducted to clarify the taxonomic status of Bacteroides type A strains with high vitamin B₁₂-producing ability that is widely distributed in the intestinal tracts of freshwater fish. Seventeen strains of Bacteroides type A isolated from five fish species were all rod-shaped and gram-negative. The strains were positive for esculin hydrolysis, nitrate reduction, resistance to bile, acid phosphatase, and negative for the production of catalase and urease and the susceptibility to vancomycin. The G+C content of DNA from the 17 strains was 29·1-31·9 mol%, and 16S rDNA sequence analysis revealed a close phylogenetic relationship between Bacteroides type A strains and Cetobacterium somerae sharing 99·7-100% sequence similarity. In addition, strains were capable of producing vitamin B₁₂ at a rate of 1·82-13·98 ng ml⁻¹ in 48 h. Phenotypic and phylogenetic characteristics indicated that all isolates previously classified as Bacteroides type A strains belong to C. someare. This study provided the important finding of novel niche of vancomycin-resistant bacteria such as C. somerae in the intestinal tract of freshwater fish.
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