Nematicidal activity of a water soluble lectin from seeds of Moringa oleifera
2018
de Medeiros, Mário Luan Silva | de Moura, Maiara Celine | Napoleão, Thiago Henrique | Paiva, Patrícia Maria Guedes | Coelho, Luana Cassandra Breitenbach Barroso | Bezerra, Ana Carla Diógenes Suassuna | da Silva, Michele Dalvina Correia
Gastrointestinal nematodes are the major cause of animal diseases, and the indiscriminate use of synthetic anthelmintic contributes to the development of drug resistance. Natural bioproducts are noteworthy since they have proved to be promising for alternative antiparasitic therapies. This study evaluated the in vitro effect of WSMoL (water soluble Moringa oleifera lectin) on hatching of eggs and on the development of early-stage larvae of gastrointestinal nematodes from naturally infected goats. In addition, the interference of WSMoL on activity of proteases was determined and the affinity of the lectin for glycosylated molecules of these parasites was investigated using fluorescein isothiocyanate (FITC)-labeled WSMoL. WSMoL at 250μgmL⁻¹ interfered on hatching of eggs (40.4% of not hatched eggs; p=0.018), and on larval development (stages L1–L3) (IC₅₀ of 78.22μgmL⁻¹). The activity of secreted proteases showed a significant increase in the presence of WSMoL (307.14U/mg–1031U/mg). FITC-labeled WSMoL recognized embryonic egg content and larval content after hatching, which suggests that WSMoL interact with intestinal glycoconjugate receptors in the embryo, as well as in cuticle of the larvae.
اظهر المزيد [+] اقل [-]الكلمات المفتاحية الخاصة بالمكنز الزراعي (أجروفوك)
المعلومات البيبليوغرافية
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