Raoultibacter phocaeensis sp. nov., A New Bacterium Isolated from a Patient with Recurrent Clostridioides difficile Infection
2022
Yacouba, Abdourahamane | Kuete Yimagou, Edmond | Lo, Cheikh Ibrahima | Tchoupou Saha, Ornella La Fortune | Alibar, Stephane | Fadlane, Amael | Fontanini, Anthony | Brechard, Ludivine | Raoult, Didier | Lagier, Jean-Christophe | Dubourg, Grégory
Strain Marseille-P8396ᵀ is a new species isolated from a patient with recurrent Clostridioides difficile infection. Its optimal growth condition was observed at pH of 7.5, at a temperature of 37 °C after 72 h of incubation on Columbia agar (BioMérieux, France) with 5% sheep blood, under an anaerobic atmosphere. Strain Marseille-P8396ᵀ cells are Gram-positive rods, nonspore‐forming, and nonmotile. 9-Octadecenoic acid (41.9%), hexadecanoic acid (22.5%), and 11-Octadecenoic acid (11.0%) represent the major fatty acid of strain Marseille-P8396ᵀ. The optimal growth condition of strain Marseille-P8396ᵀ was observed at 37 °C after 72 h of incubation under an anaerobic atmosphere, pH ranging from 6.5 to 8.5, and salinity of 0.5 to 7.5%. Its genome (Genbank Accession Number NZ_CABDUX000000000) size was 3.86 Mb with 59.4 mol% of G+C content, and 3,124 protein-coding genes. The 16S rRNA gene sequence (Genbank accession number NR_148574.1) of strain Marseille-P8396ᵀ shared a similarity of 98.71% with Raoultibacter timonensis strain Marseille-P3277ᵀ (Genbank accession number NR_148574.1), currently the most closely related species. However, the OrthoANI and digital DNA–DNA hybridization values with Raoultibacter timonensis strain Marseille-P3277ᵀ (Genbank accession number OEPT01000000) were 80.15% and 24.6 ± 4.8%, respectively. Taken together, these results clearly demonstrate that strain Marseille-P8396ᵀ represents a new species within the genus Raoultibacter described here as Raoultibacter phocaeensis sp. nov. (type strain: Marseille-P8396ᵀ=CSUR8396ᵀ=CECT 30202ᵀ).
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